Built for What’s Next Cell Line Development
Stable, high-producing CHO and HEK293 cell lines designed for scalable biologics manufacturing, from development through commercial supply.
Value
Build A Strong Foundation
For Your Program
Designed for performance and stability
Cell lines are selected for high productivity, consistent quality attributes, and long-term genetic stability.
Aligned with downstream manufacturing
Development is structured to support seamless transition into process development and CGMP manufacturing.
Built for regulatory readiness
Documentation and characterization are designed to support IND and future regulatory submissions.
Integrated with your full program
Cell line development connects directly to Avid’s broader development and manufacturing capabilities.
CHO AND HEK293 EXPRESSION SYSTEMS
The Right System
for Your Molecule
CHO cell lines: Industry-standard platform for monoclonal antibodies and many recombinant proteins, optimized for high yield and regulatory familiarity.
HEK293 cell lines: Ideal for complex proteins requiring human-like post-translational modifications and specific glycosylation patterns.
PLATFORM TECHNOLOGY
Optimized for Speed
& Predictability
Avid leverages advanced cell line development platforms designed to accelerate clone selection, improve expression efficiency, and support consistent results across programs.
These platforms integrate seamlessly with downstream development and manufacturing workflows, ensuring a smooth transition into the next phase of your program.
BUILT FOR CONSISTENT PERFORMANCE
Designed to Support Every Stage
High productivity
Clones are selected for strong expression levels to support efficient manufacturing.
Product quality alignment
Cell lines are evaluated for critical quality attributes early in development.
Genetic stability
Long-term stability ensures consistent performance across manufacturing campaigns.
Scalability
Development is aligned with downstream processes to support smooth scale-up.
Our Process
From Gene to Banked Cell Line
-
01
Gene construct review & transfection
Evaluation of your construct and introduction into CHO or HEK293 host cells.
-
02
High-throughput clone screening
Selection of high-performing clones based on productivity and product quality.
-
03
Monoclonality confirmation
Single-cell cloning and documentation supporting regulatory requirements.
-
04
Stability assessment
Verification of long-term productivity and genetic consistency.
-
05
Cell banking
Generation of research cell bank (RCB) and GMP-compliant master cell bank (MCB).
Deliverables
Designed for
Development and Beyond
Research Cell Bank (RCB): Cell stock supporting early development and feasibility work.
Master Cell Bank (MCB): Fully characterized, GMP-compliant cell bank for clinical and commercial manufacturing.
Monoclonality documentation: Regulatory-ready evidence supporting single-cell origin.
Stability data package: Data demonstrating consistent performance over time.
Product quality characterization: Assessment of key quality attributes from selected clones.
CMC-ready documentation: Structured records supporting IND and BLA submissions.
APPROACHES
Built to Scale
Cell line development is not a standalone step, it’s the starting point for everything that follows.
At Avid, your cell line is developed within the same environment that supports process development, analytical testing, and manufacturing. This continuity helps ensure consistency, efficiency, and alignment as your program advances.
Why Us
Why Avid Bioservices
30+ years of Biologics Expertise
Deep experience across development and manufacturing.
End-to-End Continuity
Direct connection from cell line development to manufacturing.
Integrated Teams
Scientists working collaboratively across disciplines.
Regulatory-Ready Approach
Documentation built to support submissions from the start.
US-Based Operations
Domestic development and manufacturing in FDA-experienced facilities.
Flexible Engagement Models
Standalone CLD or fully integrated programs.
Start your cell line
development program
Tell us about your molecule. We’ll design a program to support your path from development through manufacturing.
Learn More
Cell Line Development FAQs
Cell line development typically takes 4–9 months depending on molecule complexity and program scope. Platform-based approaches can support efficient timelines while maintaining regulatory rigor.
A research cell bank (RCB) supports early development work, while a master cell bank (MCB) is a fully characterized, GMP-compliant source used for clinical and commercial manufacturing.
CHO is the standard for most biologics, particularly monoclonal antibodies. HEK293 is often used for proteins requiring specific human-like modifications. Selection depends on your molecule’s characteristics.
CLD programs generate monoclonality data, stability data, cell characterization, and cell banking documentation , all supporting the CMC section of an IND.
Yes. Avid provides integrated services including process development, analytical development, and CGMP manufacturing.
US-based development supports regulatory alignment, supply chain reliability, and streamlined communication throughout the program lifecycle.